Urine Luck! – A Urinalysis Simulation


Students examine urine samples from patients and suggest a diagnosis and treatment plan  based on test results. Patient charts and history are included so that students can use background information plus the results of tests to diagnosis the patient.

Urine can be made from basic chemicals, mainly apple juice, food coloring, water, vinegar, and sodium hydroxide to create a basic solution.  Students observe physical characteristics, such as smell and color and then perform tests to determine if the urine has sugar or proteins. 

Students also use test strips to simulate a test to establish the presence of ketones and human chorionic gonadotropin.

In the final analysis section, students fill out a CER (claim, evidence, reasoning) to provide a diagnosis for their patient and a possible treatment plan.

Instructions for five patients are included, though I would suggest giving groups only 1 (or 2) patients to test just to save time and expense of materials.   Also, it reduces cheating when groups know that their patient will have different results than other groups.

Materials Needed: 

Water, food coloring, egg whites (proteins), apple juice, 

HCL, NaOH, Ammonia – you can add other substances to make your urine look more realistic. 

Recommend making a large stock solution and then distributing to groups in specimen jars or beakers. Test stock to make sure desired test results occur.

You will also need additional materials to perform the sugar test – Benedict’s solution needs to react in a warm water bath.  Alternatively, you could buy glucose test strips.   

Biuret reagent is needed to perform the protein test, but it does not need a warm water bath.  pH paper is used to simulate the pregnancy and ketone tests.   You can also get Urine test strips which test Glucose, pH, and proteins which will reduce your set-up time.

Grade Level:  10-12
Time Required:   30-45 minutes  (more if you have students test all patients)