This AP Biology Lab requires students to set up respirometers to measure the oxygen consumption of germinating peas in cold and warm water. Students can complete the lab in two days – 1 day for setup and data collection and the second day for a discussion and analysis of the data.
Lab guide also includes a discussion of cellular respiration, though it may be best to conduct this investigation after a lecture on cellular respiration. As a pre-lab, students can also watch the video by BozemanScience that outlines the set-up and procedure.
Most of the equipment can be reused year after year. I have permanently affixed the pipets to the rubber stoppers and sealed with gorilla glue, which eliminates one major problem with the lab where the pipets leak. Test tubes can be substituted for glass vials, though you might need to reduce the overall volumes. Potassium hydroxide can probably be found in the chemistry department, though you don’t need very much of it, it is essential for this lab to work properly.
Grade Level: 11-12, Advanced Placement Biology
Time Required: 1-2 Class Periods