The Tardigrade Project

Tardigrades belong to the Animal Kingdom in the phylum tardigrada. They are small, multicellular heterotrophs that are found almost everywhere in the world, hiding almost in plain sight. They are small creatures, measuring about 1.5 mm (1500 micrometers) in length

They have a unique ability to survive extreme conditions by shedding all of the water and curling up into a structure called a "tun." Tardigrades have even been known to survive in space! In this project, you will collect tardigrades from the environment and view them with a microscope, collect data and complete a project about tardigrades.

Collecting Your Tardigrades

Tardigrades can be collected from lichens growing on trees.    Lichens will appear as green or white masses on the bark of a tree.  Lichens are actually a symbiotic relationship between algae and fungus and they can serve as a habitat for many microorganisms, including tardigrades.

Using a scalpel or other tool (paint scraper, butter knife, screwdriver) to scrape lichens from the tree. You do not want the entire layer of bark, just the part that has the lichens.

Store your samples in a paper bag.    You may want to get multiple samples from different areas, using the bag to label each location and describe the appearance of the lichen. This may be important information if your experimental question relates to where tardigrades are located.

Filtering Tardigrades Using Baermann Pans

baermann plate

Baermann pans can be constructed from foam dinner plates, tulle and staples.  The general idea is that two plates are sandwiched, and a sample is placed over the mesh screen (tulle) where the microorganisms will fall down into the pan below.  This method works for collecting microorganisms like nematodes, rotifers, amoebas, and tardigrades.

check box 1.  Cut a hole in one plate and cover it with a nylon mesh or tulle. Staple to hold the mesh into place. You can also opt to trim away any of the loose mesh.  *These may have been made for you already.
check box 2.  Lay a thin (single ply) tissue over the screen.
check box 3.  Place the plate with mesh over another plate that does not have mesh (like a sandwich).

check box 4.  Place your sample of lichens over the mesh and pour a generous amount of distilled water over the top. This water will filter to the plate below and over the course of 12-24 hours, microorganisms will also move into the lower pan.

check box 5. Your teacher may check your pans in a couple of hours, if there is very little water, more will be added, the tissue should stay moist.  Placing another plate over the top one may reduce evaporation. 

check box 6. DO NOT DISTURB the pans.

-------------------------------------- 24 Hours Later  ----------------------------------

check box 1. Lift the sample and tilt into the lower plate to speed drainage.
check box 2.  Carefully pour the water from the lower plater into a beaker (or cup)
check box 3.  Use distilled water to rinse the plate, many microorganisms will cling to the bottom. You want to wash them into the beaker.

check box 4.  Use a pipette to draw water from the bottom of the beaker where more of your microorganisms will be located.  Place in a petri dish and use a dissecting scope to scan the sample for evidence of life.

check box 5.  Be patient, tardigrades are easily overlooked!   You may also find small worms called nematodes, rotifers, and other living things in your sample. 

check box 6.  If you can find one using the dissecting scope, the pipette can be used to carefully transfer it to a microscope slide and view at a higher power.  Do not use coverslips (you’ll smush your tardigrade!) and only view with the scanning (40x) and low power objective (100x).

 

Things to do with your Tardigrades - Your Project

Hopefully you have found a tardigrade and maybe even other living organisms in your sample.   Choose ONE of the following tasks to gather data and prepare a report, photo, or video.

1.  Consider your experimental question from your research project.   Use your tardigrades to answer the question and write a report.  *This might be difficult giving constraints of laboratory equipment and time.

2.  Compare locations and samples.  If you didn’t find tardigrades in your sample, check out other groups and gather evidence about differences between sampling and filtering techniques.  Write your report on the best way to get tardigrades. 

3.  Expose your tardigrade to different environments and observe its behavior.  Does it respond to light?  Does it respond to other tardigrades?   Write a report that summarizes the observed behaviors of the tardigrade.

4.  Take a photo of your tardigrade and label any anatomical features you can with captions about their functions.  Share this with the class.

5.  Take a video of your tardigrade and narrate it with “fun facts” about tardigrades.    Share this with the class.