Objectives: In this simulation you will examine crime scene evidence to determine who is responsible for eating the Queen's special imported Lindbergher Cheese (yes, the stinky cheese). You will model the process of electrophoresis and DNA fingerprinting.
ROYAL GUARD INCIDENT REPORT
Method of Entry: Unknown, no evidence of force on doors or windows.
Narrative: The cheese was allegedly stolen from the Queen's sitting room the night before the grande ball. The cheese was listed as a gift from the Manchurian diplomat. Officer Li Gase dusted for fingerprints and found none on the table or doors, the maid claimed that they had been wiped clean earlier. The wheel of cheese was on a platform in the sitting room, and half of it had been eaten. We took pictures of the half eaten cheese and sent it to the lab for further tests. Edna N. Zime, the lab technician said that saliva samples could be taken from the teeth imprints of the cheese that was left behind.
Suspect Number: 1
Suspect Number 2
Suspect Number 3
Suspect Number 4
Crime Lab Data
Narrative: After receiving the package with the plastic bag marked Crime Scene, the DNA was extracted. Because the sample was so small, the DNA was amplified using the polymerase chain reaction. We isolated the DNA from the four suspects and compared them to the crime scene DNA using DNA restriction analysis.
Results: See attached DNA Results
1. Turn your paper strips (DNA sequences) so that the side with the bases is facing you. The restriction enzyme cuts at each point it finds C C G G, always cutting between the C and the G. Label the back of the slips with the suspect number so that you don't get them confused after cutting. Use scissors to cut the DNA sequence at the C C G G points.
2. Count the number of base pairs (bp) in each piece of DNA that you created. Record the base pair number on the back side of the DNA fragment.
3. Make an enlarged chart like the one shown. Your teacher will give you paper for this. Use a ruler to ensure that the lengths are uniform. Alternatively, you can use chalk markers on the lab desk.
4. Tape your DNA fragments to the chart, using the base pair numbers as a guideline for fragment placement.
5. Compare the crime scene DNA to the suspects and indicate on your chart, which suspect is guilty of eating the cheese.
1. On your chart, label the positive (+) and the negative (-) ends. Circle the suspect's DNA who matches the DNA at the crime scene and write the name of the suspect.
Who ate the cheese? ___________________________________________
How do you know? _________________________________________________
2. For each of the following tasks performed in the activity, describe what they are actually simulating.
Cutting the DNA into fragments: ________________________________________________________
Taping the DNA onto the large paper: _____________________________________________________
3. For each word below, describe how it relates to DNA Fingerprinting:
Polymerase Chain Reaction __________________________________________________________________
Gel Electrophoresis ________________________________________________________________________
Restriction Enzyme _________________________________________________________________________