Enzyme Lab - Teachers Guide

Original Document: Enzyme Lab

MATERIALS: The chemicals can be purchased from chemical or biological supply companies, students should wear safety goggles This lab requires some set up and can be messy. Not advised unless you actually have a lab to work within.


6 Test tubes
Test tube holders
3% Hydrogen peroxide

Straight-edged razor blade 
Scissors and Forceps
Measuring Pipettes
Stirring rod 

Fresh liver, Apple, and Potato, Yeast
Vinegar / Baking Soda
HCL and NaOH
pH paper (optional)

Ice bath
Warm water bath
Boiling water bath

The majority of the synthesis questions use the Claim - Evidence - Reasoning model. This rubric can be used to grade each of them. I print small label stickers to projects to make grading quick. Students in my class are used to this model by the time we do this lab.

Label Version (Print on Labels for exams)

Claim (accurate, complete, grammar)  __3__2__1
Evidence (appropriate, sufficient, data)  __3__2__1
Reasoning (links to claim and sci. principles) 3__2__1

I do not have the answers to the questions (key) but generally speaking..

It is more important that students provide evidence from their experiment to support their claims.


  Accomplished (3) Proficient (2) Developing (1)
CLAIM Makes an accurate and complete claim, uses complete and grammatically correct sentences Makes an accurate, but incomplete claim, or grammatically incorrect Claim is not accurate, incomplete, or unintelligible
EVIDENCE Provides appropriate and sufficient evidence to support claim by referencing specific data, observations, or text evidence (for readings) Provides appropriate data but insufficient data, too general or lacking in details Provides evidence but it is insufficient, inaccurate, no details
REASONING Provides thorough reasoning that links evidence to the claim, references scientific principles that are relevant to claim Provides reasoning that links evidence to the claim, lacks scientific principles Does not provide reasoining, or reasoning does not link evidence.