Name____________________________

Karyotyping Activity
In this activity, you will use a computer model to look at chromosomes and prepare a karyotype. You will diagnose patients for abnormalities and learn the correct notation for characterizing karyotypes.

Go to www.biology.arizona.edu - click on Karyotyping under human biology.
Introduction:

1. What causes a dark band on the chromosome? _____________________________________
2. What is a centromere? _______________________________________________________

Patient Histories: *Click on Patient Histories. You will be completing a karyotype for Patient A, B & C

Patient A ( Click on the link to "Complete Patient A's Karyotype" )
*Match the chromosome to its homolog. After all the matches are complete you'll analyze your patient. (Scroll down to view your completed karyotype).

3. What is patient A's history (summarize) ______________________________________________
4. How many total chromosomes are in your karyotype - count them _________
The last set of chromosomes is the sex chromosomes, if you have two large chromosomes, your patient is XX (female), one large and one small indicates and XY (male) . What sex chromosomes does your patient have ________
Which chromosome set has an extra + _______
5. What diagnosis would you give this patient (what disease)? ______________

Patient B - click on the link to go to Patient B and repeat the above process.

6. What is Patient B's history (summarize) _______________________________________________
7. How many total chromosomes are in your karyotype - count them _________
What sex chromosomes does your patient have ________
Which chromosome set has an extra + _______
8. Finish the notation for this patient's karyotype : 47 X _____
9. What is the diagnosis? __________________________________

Patient C - click on the link to go to Patient C and repeat the above process.

10. What is patient C's history (summarize)? _________________________________________
11. How many total chromosomes are in your karyotype - count them _________
What sex chromosomes does your patient have ________
Which chromosome set has an extra + _______
12. Write out the correct notation for this karyotype. ________
13. What is the diagnosis? _________________________________________

Site 2: Genetic Science Learning Center ( http://gslc.genetics.utah.edu )
Understanding Genetic Disorders ' What Can Our Chromosomes Tell Us
(Find the answers to the following questions in this area. Browse all sections)

1. What is Giemsa? _______________________________________
2. What are the three key features used to read chromosomes?
____________________________
_____________________________
_____________________________
3. Sketch or describe: metacentric, submetacentric, acrocentric

4. Got o Making a Karyotype - Try it yourself
Create your own karyotype - turning on hints is okay. Check this box when your karyotype is complete
What did you find difficult about matching the chromosomes? _____________________________

5. Go to -Using Karyotypes to Predict Genetic Disorders
What is trisomy? ______________________________________
What is monosomy? ____________________________________
What is a terminal deletion? ____________________________________________

6. For each of the Disorders, describe the chromosome abnormality and the symptoms.

Cri Du Chat

Turner Syndrome

Klinefelter Syndrome

Williams Syndrome

Extra Credit - Do at home

Site: http://bluehawk.monmouth.edu/~bio/karyotypes.htm
Pick from the list of abnormal karyotypes and arrange the chromosomes in a karyotype. Use the "print screen" button to copy your finished karyotype onto a word processing document. For "Diagnosis" write the chromosome set that has the abnormality, and what type of abnormality it is. Print this page out and turn it in.