Bacteria Lab

Gram Stains

First of all, review the steps for Gram Staining at http://www-micro.msb.le.ac.uk/video/Gram.html

1. Who developed the Gram Stain technique?

2. Why is gram staining important for classifying bacteria?

3. Compare gram negative bacteria to gram positive bacteria.

Types of Bacteria

http://helios.bto.ed.ac.uk/bto/microbes/shape.htm

After reading the page and observing the pictures, you should be able to answer the following.

4. What are the four shapes of bacteria?

5. How are colonies analyzed from an agar plate?

6. Why are there so few colonies in the sample taken from the mouth?

Bacteria Images

http://www.meddean.luc.edu/lumen/DeptWebs/microbio/med/gram/slides.htm - Slide Show

Specimen Organisms Gram (+ or - )ShapeSketch
1. Urethral smear
2. Pus smear
Neisseria gonorrhoeae
3. Pus smear (wound) Staphylococcus aureus

4. Exudate smear
5. Tissue smear

Clostridium perfringens
6. Knee tap smear
Group A
Streptococcus pyogenes
7. Sputum smear Staphylococcus aureus
8. Sputum smear Staphylococcus & Streptococcus pneumoniae
9. Sputum smear
10. Sputum smear
11. Sputum smear
Streptococcus pneumoniae
12. Spinal fluid Haemophilus influenzae
13. Urine smear Escherichia coli/PMN
14. Peritoneal fluid Mixed anaerobic infection
15. Spinal fluid Cryptococcus neoformans
16. Skin smear Candida albicans

http://www.kcom.edu/faculty/chamberlain/Website/gallery.htm

Gram + or -
Shape
Sketch
17. Lactobacillus
18. Enterococcus
19. Bacillus anthracis
20. Staphylococcus epidermis
21. Salmonella enteriditis
22. Yersinia pestis
23. Mycobacterium leprae
24. Mycobacerium tuberculosis
25. Vibrio cholerae

Bacteria Pathogens

http://www.cdc.gov/ncidod/dbmd/ - go to disease listing - you may also want to access the CDC's main page to find information on some of these. Search before asking!

For each of the diseases listed, determine the causative agent, method of transmission and symptom

PathogenTransmissionSymptoms
Anthrax
Botulism
Cholera
E. Coli
Leprosy (Hansen's Disease)
Pneumoniae (Mycoplasma)
Salmonella (enterititis)
Strep Throat
Gonorrhea
Plague (black plague or bubonic plague)
Tetanus